Bulletin of Entomological Research (2006) 96, 1-13

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M.H. Greenstone (2006)
Molecular methods for assessing insect parasitism
Bulletin of Entomological Research 96 (1), 1-13
Abstract: Determining insect parasitism rates is problematic due to the small size and lack of useful distinguishing morphological characters of many parasitoid taxa. To solve this problem, entomologists have employed one of four general methods to detect parasitoid protein or nucleic acid markers: serological assay; random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR); allozyme electrophoresis; or specific PCR. Serological methods, especially with monoclonal antibodies, are unrivalled for specificity, enabling discrimination at the stage as well as species level. However, they have not found favour with many workers, possibly due to complexity and expense. RAPD-PCR has been widely used, but can only be recommended for restricted applications because of its poor reproducibility. Allozyme electrophoresis provides reproducible detection and discrimination of closely related species. Specific-PCR is highly specific and reproducible, and also has the shortest latency for detection, usually 24 h or less after parasitization. The substantial existing literature on allozyme electrophoresis and specific PCR is used to support recommendations on what are apt to be fruitful enzyme systems or genomic regions for detecting and discriminating parasitoids in untried parasitoid-host assemblages.
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Link to article at publishers website
Database assignments for author(s): Matthew H. Greenstone

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Nasonia vitripennis (parasitoid)
Muscidifurax raptor (parasitoid)
Trichogramma turkestanicum (parasitoid)
Trichogramma pretiosum (parasitoid)
Trichogramma minutum (parasitoid)
Lysiphlebus testaceipes (parasitoid)
Aphelinus abdominalis (parasitoid)
Aphelinus asychis (parasitoid)
Spalangia nigroaenea (parasitoid)
Spalangia cameroni (parasitoid)
Trichogramma brassicae (parasitoid)
Spalangia endius (parasitoid)
Muscidifurax zaraptor (parasitoid)
Trichogramma chilonis (parasitoid)
Aphidius colemani (parasitoid)
Anisopteromalus calandrae (parasitoid)
Aphidius ervi (parasitoid)
Trichogramma ostriniae (parasitoid)
Ageniaspis citricola (parasitoid)
Aphelinus albipodus (parasitoid)
Trichogramma dendrolimi (parasitoid)
Anaphes iole (parasitoid)
Lydella thompsoni (parasitoid)
Muscidifurax raptorellus (parasitoid)
Urolepis rufipes (parasitoid)
Cotesia congregata (parasitoid)
Aphelinus hordei (parasitoid)
Trichogramma australicum (parasitoid)
Trichomalopsis sarcophagae (parasitoid)
Peristenus digoneutis (parasitoid)
Leiophron uniformis (parasitoid)
Peristenus howardi (parasitoid)
Peristenus pallipes (parasitoid)
Peristenus mellipes (parasitoid)
Trichogramma deion (parasitoid)
Dolichogenidea tasmanica (parasitoid)
Aphelinus varipes (parasitoid)
Aphelinus gossypii (parasitoid)
Lipolexis oregmae (parasitoid)
Peristenus relictus (parasitoid)
Leiophron argentinensis (parasitoid)