Bulletin of Entomological Research (2000) 90, 317-327

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A. Kirk, L.A. Lacey, J.K. Brown, M.A. Ciomperlik, J.A. Goolsby, D.C. Vacek, L.E. Wendel and B. Napompeth (2000)
Variation in the Bemisia tabaci s. l. species complex (Hemiptera: Aleyrodidae) and its natural enemies leading to successful biological control of Bemisia biotype B in the USA
Bulletin of Entomological Research 90 (4), 317-327
Abstract: Parasitoids of the Bemisia tabaci (Gennadius) species complex collected in Spain and Thailand were evaluated as biological control agents of B. tabacibiotype B in cole crops in Texas, USA. Parasitoids were identified by morphological and RAPD-PCR analyses. The most abundant parasitoid from Spain was Eretmocerus mundus Mercet with apparent field parasitism of 39-44%. In Thailand, Encarsia formosa Gahan, E. transvena Timberlake, E. adrianaeLopez-Avila, Eretmocerus sp. 1 and sp. 2 emerged, with apparent field parasitism of 1-65%. Identification and molecular classification of B. tabaciassociated with parasitoid collections and in the release site in Texas were accomplished using morphological traits and nucleotide sequence comparison of the mitochondrial cytochrome oxidase I gene (COI) (700-720 bp). Collections of B. tabacifrom Thailand grouped separately from B types from Arizona and Florida and the target B type from Texas, USA, a cluster from India, and other New World B. tabaci. The Spanish B. tabaci host of E. mundus which was laboratory and field-tested to achieve biological control of the B type was most closely related to non-B type B. tabaci populations from Spain and Sudan, the latter which formed a second group within the larger clade that also contained the B type cluster. Laboratory tests indicated that E. mundus from Spain parasitized more B. tabaci type B than did Eretmocerus spp. native to Texas and other exotic parasitoids evaluated. Eretmocerus mundus from Spain also successfully parasitized B. tabaci type B when field-released in a 0.94 million ha test area in Texas, and has significantly enhanced control of B. tabaci type B in California, USA. In contrast, parasitoids from Thailand failed to establish in the field in Texas, collectively suggesting a positive correlation between the centres of diversity of compatible parasitoid-host complexes.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Lawrence A. Lacey, John A. Goolsby, Alan A. Kirk, Matthew A. Ciomperlik

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Bemisia tabaci biotype MEAM1 U.S.A. (mid S)
Bemisia tabaci Spain (continental)
Bemisia tabaci Thailand
Encarsia formosa (parasitoid) Bemisia tabaci Thailand
Encarsia sophia (parasitoid) Bemisia tabaci Thailand
Eretmocerus mundus (parasitoid) Bemisia tabaci biotype MEAM1 U.S.A. (mid S)
Eretmocerus mundus (parasitoid) Bemisia tabaci Spain (continental)
Encarsia azimi (parasitoid) Bemisia tabaci Thailand