Biocontrol Science and Technology (1998) 8, 45-57

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I.A. Khmel, T.A. Sorokina, N.B. Lemanova, V.A. Lipasova, O.Z. Metlitski, T.V. Burdeinaya and L.S. Chernin (1998)
Biological control of crown gall in grapevine and raspberry by two Pseudomonas spp. with a wide spectrum of antagonistic activity
Biocontrol Science and Technology 8 (1), 45-57
Abstract: Pseudomonas aureofasciens B-4117 and P. fluorescens CR330D inhibited the growth of a wide range of plant pathogens, including Agrobacterium tumefaciens, when tested on agar media. In a series of nursery-based trials with natural pathogen inoculum, application of either B-4117 or CR330D significantly reduced the incidence and severity of crown gall caused by A. tumefaciens on grapevine and raspberry. The extent of disease control depended upon the variety tested. Both bacteria reduced disease during seedling root production and grafting. The disease incidence on root cuttings of three grapevine varieties was reduced by 56-80% and the disease severity index (DSI) was decreased by 75-86%. Depending on the scion variety, the number of healthy rooted grafts increased by 2-3.5-fold, while the DSI was reduced by 1.5-3-fold. The results suggest that there is potential in using these antagonists to diminish the influence of latent rootstock infection on graft sensitivity to crown gall. Pretreatment of rooted raspberry seedlings with P. aureofaciens B-4117 prevented the development of crown galls caused by A. tumefaciens strain K24 or by a mixture of A. tumefaciens pathogenic strains previously isolated from raspberry. Both Pseudomonas spp. persisted on the root surfaces of inoculated vine cuttings and in non-sterile soil. The advantages of using the antagonistic bacteria as biocontrol agents of crown gall are discussed.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website


Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution
evaluation - screening - selection


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Agrobacterium tumefaciens Raspberry/blackberry (Rubus)
Agrobacterium tumefaciens Grapevine (Vitis)
Pseudomonas fluorescens (antagonist) Agrobacterium tumefaciens Raspberry/blackberry (Rubus)
Pseudomonas fluorescens (antagonist) Agrobacterium tumefaciens Grapevine (Vitis)
Pseudomonas chlororaphis (antagonist) Agrobacterium tumefaciens Raspberry/blackberry (Rubus)
Pseudomonas chlororaphis (antagonist) Agrobacterium tumefaciens Grapevine (Vitis)