BioControl (2002) 47, 685-696

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R. Crespo, M.P. Júrez, G.M. Dal Bello, S. Padín, G. Calderón Fernández and N. Pedrini (2002)
Increased mortality of Acanthoscelides obtectus by alkane-grown Beauveria bassiana
BioControl 47 (6), 685-696
Abstract: The effect of alkane-growth induction of the entomopathogenic fungus Beauveria bassiana (Balsamo) Vuillemin (Deuteromycotina: Hyphomycetes), on the ability to kill the bean weevil Acanthoscelides obtectus Say (Coleoptera: Bruchidae), was tested. Adult insects were sprayed with an 0.01% Tween 20 aqueous suspension of 4 × 106 conidia/ml. The performance of fungi grown in complete agar medium containing glucose as carbon source (FS0) was compared to that of alkane-grown fungi (FS1) with n-hexadecane as the only carbon source. Mortality increased (p < 0.05) from 22 ± 4.5% to 44 ± 11.4% at day 7, and from 26 ± 5.5% to 60 ± 7.1% 14 days after treatment with FS0 or FS1 respectively. The insect epicuticular hydrocarbons were analysed by capillary gas chromatography (CGC); major components were saturated hydrocarbons, 27 to 29 carbons in length. A variety of methyl-branched isomers of C27 were the prevailing structures, and nC27 was the major straight chain component. Whole insect hydrocarbons were qualitatively identical to those of the epicuticular surface. Oleic, linoleic and palmitic acids accounted for almost 88% of the fungal fatty acids, irrespective of the carbon source used for growth; however, the unsaturated/saturated ratio diminished markedly from 4.32 (FS0) to 2.47 (FS1). These results indicate that alkane supplementation of culture media might be a tool to improve the virulence of some mycoinsecticides.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): M. Patricia Juarez, Gustavo M. Dal Bello

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
rearing/culturing/mass production


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Acanthoscelides obtectus
Beauveria bassiana (entomopathogen) Acanthoscelides obtectus