Australian Journal of Agricultural Research (1999) 50, 333-342

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A. Padovan, S. De La Rue, R. Eichner, R. Davis, A. Bernuetz, B. Schneider and K. Gibb (1999)
Detection and differentiation of phytoplasmas in Australia: an update
Australian Journal of Agricultural Research 50 (3), 333-342
Abstract: Phytoplasmas were found in 33 plant species that were not described as host plants in an earlier Australian survey. Plants displayed characteristic symptoms of little leaf, proliferation, and floral abnormalities. Restriction fragment length polymorphism analysis revealed 13 different restriction patterns. The majority of phytoplasmas showed a restriction pattern identical to that of either the tomato big bud (TBB) or sweet potato little leaf V4 (SPLL-V4) phytoplasma. Phytoplasmas from 6 plant species showed a restriction pattern similar to that of the pigeonpea little leaf (PLL) phytoplasma. One phytoplasma from garden bean displayed a restriction pattern identical to that found in papaya dieback and Australian grapevine yellows (AGY) phytoplasmas. Seven new restriction fragment patterns have been detected and sequence analysis of the 16S/23S spacer region revealed that 3 of these phytoplasmas are related to the faba bean phyllody (FBP) group. The spacer region of a graminaceous phytoplasma was most similar to phytoplasmas from the sugarcane white leaf group. Another graminaceous phytoplasma was identical to a phytoplasma from Indonesia. The spacer region of a phytoplasma from poinsettia (PoiBI) was identical to the western X-disease phytoplasma from North America and Europe. The spacer region of a phytoplasma in stylosanthes contained no tRNAIle. Full-length 16S rRNA gene sequences from selected new phytoplasmas were determined to corroborate results obtained from the spacer region analyses. Three of these phytoplasmas (galactia little leaf, vigna little leaf, and stylosanthes little leaf) are, along with the PoiBI phytoplasma and the graminaceous phytoplasmas, members of phytoplasma groups that have not been reported before in Australia.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Bernd Schneider, Anna Padovan

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution
identification/taxonomy


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Phytoplasma australiense
Phytoplasma pruni
Sugarcane white leaf phytoplasma
Sorghum grassy shoot phytoplasma Australia (Western)
Vigna little leaf phytoplasma Australia (NT+QLD)
Phytoplasma aurantifolia Australia (NT+QLD)