Australasian Plant Pathology (2003) 32, 511-519

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Alexander Idnurm, Janet L. Taylor, M. Soledade C. Pedras and Barbara J. Howlett (2003)
Small scale functional genomics of the blackleg fungus, Leptosphaeria maculans: analysis of a 38 kb region
Australasian Plant Pathology 32 (4), 511-519
Abstract: A small-scale, functional genomics approach was applied to Leptosphaeria maculans, the dothideomycete that causes blackleg disease of oilseed Brassicas. Analysis of 126 Expressed Sequence Tags identified a putative Zn[II]2Cys6 gene, znr1, most similar to aflR, the gene regulating aflatoxin production in Aspergillus species. Eight other genes were identified within a 38.1 kb region of a cosmid clone surrounding this gene. A novel monomodular amino acyl activator gene (maa1), most similar to bacterial non-ribosomal peptide synthetases involved in antibiotic and toxin production was adjacent to znr1. Additionally, homologues for genes encoding a major facilitator transporter, a DNA mismatch repair protein and an endo/exoxylanase found in other organisms in clusters of functionally related genes involved in antibiotic or toxin production were present on the clone. The linkage of these genes suggested that they may function in metabolite production in L. maculans. Nevertheless, targeted disruption of znr1 and maa1 did not result in any alteration in growth in vitro, metabolite production, pathogenicity on Brassica napus or B. juncea, or changes in transcription of the other genes on the cosmid.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Barbara J. Howlett, M. Soledade C. Pedras

Research topic(s) for pests/diseases/weeds:
molecular biology - genes


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Plenodomus lingam Rape/canola (Brassica napus)