Archives of Virology (2019) 164, 1453-1457
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A rapid, sensitive and inexpensive method for detection of Grapevine red blotch virus without tissue extraction using loop-mediated isothermal amplification
Archives of Virology 164 (5), 1453-1457
Abstract: Grapevine red blotch virus (GRBV) is an emerging virus of significant viticultural importance throughout North America. Here, we report the development of a simple protocol for point-of-use detection of GRBV. Extraction of nucleic acids is not required; instead, the whole intact plant can simply be pricked with a sterile pipette tip, which is then incubated in sterile distilled water to provide the sample template in a loop-mediated isothermal amplification (LAMP) reaction. This method is 10,000 times more sensitive than conventional PCR, costs under a dollar per sample, and can be completed from sampling to readout in just over half an hour.
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|Grapevine red blotch virus||Grapevine (Vitis)|