Archives of Virology (2019) 164, 1453-1457

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J. Lucina Romero Romero, Gavriela Dena Carver, Patricio Arce Johnson, Keith L. Perry and Jeremy R. Thompson (2019)
A rapid, sensitive and inexpensive method for detection of Grapevine red blotch virus without tissue extraction using loop-mediated isothermal amplification
Archives of Virology 164 (5), 1453-1457
Abstract: Grapevine red blotch virus (GRBV) is an emerging virus of significant viticultural importance throughout North America. Here, we report the development of a simple protocol for point-of-use detection of GRBV. Extraction of nucleic acids is not required; instead, the whole intact plant can simply be pricked with a sterile pipette tip, which is then incubated in sterile distilled water to provide the sample template in a loop-mediated isothermal amplification (LAMP) reaction. This method is 10,000 times more sensitive than conventional PCR, costs under a dollar per sample, and can be completed from sampling to readout in just over half an hour.
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Database assignments for author(s): Keith L. Perry, Jeremy R. Thompson

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.
Grapevine red blotch virus Grapevine (Vitis)