Archives of Virology (2015) 160, 3075-3078

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Beata Hasiów-Jaroszewska, Daria Budzynska, Natasza Borodynko and Henryk Pospieszny (2015)
Rapid detection of genetically diverse Tomato black ring virus isolates using reverse transcription loop-mediated isothermal amplification
Archives of Virology 160 (12), 3075-3078
Abstract: A reverse transcription loop-mediated isothermal amplification assay (RT-LAMP) has been developed for detection of tomato black ring virus (TBRV) isolates collected from different hosts. One-step RT-LAMP was performed with a set of four primers, the design of which was based on the coat protein gene. Results of RT-LAMP were visualized by direct staining of products with fluorescent dyes, agarose gel electrophoresis, and analysis of amplification curves. The sensitivity of RT-LAMP was 100-fold greater than that of RT-PCR. The RT-LAMP assay developed here is a useful and practical method for diagnosis of TBRV.
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Database assignments for author(s): Beata Hasiów-Jaroszewska, Henryk Pospieszny, Natasza Borodynko

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Tomato black ring virus Poland