Archives of Virology (2014) 159, 353-358

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Rugang Li, Zhangjun Fei and Kai-Shu Ling (2014)
Molecular and biological properties of Tomato necrotic stunt virus and development of a sensitive real-time RT-PCR assay
Archives of Virology 159 (2), 353-358
Abstract: The newly identified tomato necrotic stunt virus (ToNStV), with a genome of 10,057 nucleotides, had 60 % nucleotide sequence and 70 % coat protein amino acid sequence identity to other potyviruses. Analysis of a second ToNStV isolate revealed two single nucleotide substitutions. ToNStV induced stunting and chlorotic to necrotic leaf symptoms on tomato plants. In host-range assays, ToNStV systemically infected many species in the family Solanaceae and locally infected some species in the families Amaranthaceae, Brassicaceae and Cucurbitaceae. A sensitive and reliable real-time RT-PCR assay was developed using simply prepared crude tissue extract.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Rugang Li, Kai-Shu Ling

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Tomato necrotic stunt virus Tomato (Solanum lycopersicum)