Archives of Virology (2009) 154, 853-856

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Beata Hasiów-Jaroszewska, Natasza Borodynko and Henryk Pospieszny (2009)
Infectious RNA transcripts derived from cloned cDNA of a Pepino mosaic virus isolate
Archives of Virology 154 (5), 853-856
Abstract: For the first time, a full-length cDNA clone of the RNA genome of pepino mosaic virus (PepMV) was constructed. RNA was extracted from purified virions of isolate PepMV-Pa and used for cDNA synthesis. The full-length cDNA was produced as one 6.4-kb fragment representing the entire PepMV genome. This fragment was ligated into the pCR® -XL-TOPO® vector downstream of T7 RNA polymerase promoter, which was included in the 5' primer sequence used for RT-PCR. The PepMV-Pa RNA transcripts obtained were infectious in different host plants, causing symptoms indistinguishable from those of the wild-type isolate. The presence and authenticity of the progeny virus were verified by ELISA, RT-PCR and nucleotide sequencing.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Beata Hasiów-Jaroszewska, Natasza Borodynko, Henryk Pospieszny

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Pepino mosaic virus