Archives of Virology (2008) 153, 1527-1535

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Sally Hilton and Doreen Winstanley (2008)
The origins of replication of granuloviruses
Archives of Virology 153 (8), 1527-1535
Abstract: The genomes of eight granuloviruses (GVs), have been analyzed for the presence of homologous regions (hrs) that may act as origins of replication. Thirteen 74-76-bp palindromes within 11 hrs have previously been identified in the Cydia pomonella GV (CpGV) genome and found to replicate in an infection-dependent DNA replication assay. We report a further palindrome within one of the hrs, which was found to replicate, bringing the total to 14 palindromes. We also report imperfect palindromes, with similar 13-bp end sequences to the CpGV palindromes, within the Adoxophyes orana GV, Cryptophlebia leucotreta GV (CrleGV), Choristoneura occidentalis GV and Phthorimaea operculella GV genomes. No hrs were detected in Agrotis segetum GV, and no additional hrs or palindromes, other than those published, were detected in the Plutella xylostella GV and Xestia c-nigrum GV genomes. Several putative hrs from the GVs were tested for replication in C. pomonella cells using a CpGV-dependent replication assay. Two CrleGV hrs were found to replicate at a low level.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website


Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Cydia pomonella granulovirus (entomopathogen)
Adoxophyes orana granulovirus (entomopathogen)
Phthorimaea operculella granulovirus (entomopathogen)
Xestia c-nigrum granulovirus (entomopathogen)
Plutella xylostella granulovirus (entomopathogen)
Cryptophlebia leucotreta granulovirus (entomopathogen)
Agrotis segetum GV (entomopathogen)
Choristoneura occidentalis granulovirus (entomopathogen)