Archives of Phytopathology and Plant Protection (2014) 47, 2493-2500

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Kiomars Rouhrazi and Heshmat Rahimian (2014)
Biochemical and genetic characterisation of Agrobacterium tumefaciens the causal agent of walnut crown gall disease in Iran
Archives of Phytopathology and Plant Protection 47 (20), 2493-2500
Abstract: In 2009-2010, crown tumours were collected from walnut (Juglans regia L.) trees in northern Iran. Gram-negative, rod shaped and aerobic bacteria with circular, convex and white-coloured colonies on potato dextrose agar plus CaCO3 medium were isolated from galls. In pathogenicity tests, tomato seedlings were inoculated with all strains and tumours started to appear three weeks after inoculation. Strains yielded a 224 bp amplicon from the virD2 gene in PCR. When the 16S rRNA gene sequence of strains was compared by BLASTn with nucleotide sequences from GenBank, it showed 99.6% identity with the 16S rRNA sequence of Agrobacterium tumefaciens ATCC 33970. Based on phenotypic and genotypic properties, the bacterium that causes crown gall of walnut trees was identified as A. tumefaciens.
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Link to article at publishers website
Database assignments for author(s): Kiomars Rouhrazi

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Agrobacterium tumefaciens Walnut/butternut (Juglans) Iran