Archives of Microbiology (2014) 196, 891-899

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Emadeldeen Ismail, Jochen Blom, Alain Bultreys, Milan Ivanovic, Aleksa Obradovic, Joop van Doorn, Maria Bergsma-Vlami, Martine Maes, Anne Willems, Brion Duffy, Virginia O. Stockwell, Theo H.M. Smits and Joanna Pulawska (2014)
A novel plasmid pEA68 of Erwinia amylovora and the description of a new family of plasmids
Archives of Microbiology 196 (12), 891-899
Abstract: Recent genome analysis of Erwinia amylovora, the causal agent of fire blight disease on Rosaceae, has shown that the chromosome is highly conserved among strains and that plasmids are the principal source of genomic diversity. A new circular plasmid, pEA68, was found in E. amylovora strain 692 (LMG 28361), isolated in Poland from Sorbus (mountain ash) with fire blight symptoms. Annotation of the 68,763-bp IncFIIa-type plasmid revealed that it contains 79 predicted CDS, among which two operons (tra, pil) are associated with mobility. The plasmid is maintained stably in E. amylovora and does not possess genes associated with antibiotic resistance or known virulence genes. Curing E. amylovora strain 692 of pEA68 did not influence its virulence in apple shoots nor amylovoran synthesis. Of 488 strains of E. amylovora from seventeen countries, pEA68 was only found in two additional strains from Belgium. Although the spread of pEA68 is currently limited to Europe, pEA68 comprises, together with pEA72 and pEA78 both found in North America, a new plasmid family that spans two continents.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Martine Maes, Maria Bergsma-Vlami, Virginia O. Stockwell, Joanna Pulawska, Aleksa Obradovic, Alain Bultreys

Research topic(s) for pests/diseases/weeds:
molecular biology - genes


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Erwinia amylovora