Applied and Environmental Microbiology (2007) 73, 337-340

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Iñigo Ruiz de Escudero, Anna Estela, Baltasar Escriche and Primitivo Caballero (2007)
Potential of the Bacillus thuringiensis toxin reservoir for the control of Lobesia botrana (Lepidoptera: Tortricidae), a major pest of grape plants
Applied and Environmental Microbiology 73 (1), 337-340
Abstract: The potential of Bacillus thuringiensis Cry proteins to control the grape pest Lobesia botrana was explored by testing first-instar larvae with Cry proteins belonging to the Cry1, Cry2, and Cry9 groups selected for their documented activities against Lepidoptera. Cry9Ca, a toxin from B. thuringiensis, was the protein most toxic to L. botrana larvae, followed in decreasing order by Cry2Ab, Cry1Ab, Cry2Aa, and Cry1Ia7, with 50% lethal concentration values of 0.09, 0.1, 1.4, 3.2, and 8.5 µg/ml of diet, respectively. In contrast, Cry1Fa and Cry1JA were not active at the assayed concentration (100 µg/ml). In vitro binding and competition experiments showed that none of the toxins tested (Cry1Ia, Cry2Aa, Cry2Ab, and Cry9C) shared binding sites with Cry1Ab. We conclude that either Cry1Ia or Cry9C could be used in combination with Cry1Ab to control this pest, either as the active components of B. thuringiensis sprays or expressed together in transgenic plants.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Primitivo Caballero, Baltasar Escriche

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
evaluation - screening - selection


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Lobesia botrana Grapevine (Vitis)
Bacillus thuringiensis Cry1A-toxin (entomopathogen) Lobesia botrana Grapevine (Vitis)
Bacillus thuringiensis Cry2A-toxin (entomopathogen) Lobesia botrana Grapevine (Vitis)
Bacillus thuringiensis Cry9C-toxin (entomopathogen) Lobesia botrana Grapevine (Vitis)
Bacillus thuringiensis Cry1I-toxin (entomopathogen) Lobesia botrana Grapevine (Vitis)