Applied Entomology and Zoology (2017) 52, 241-246

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En-Tao Sun, Yi-Nan Wang, Kang Wang, Shu-Jun Xu, Dan-Yang Nan, Wen-Jie Chen and Ying-Ying Zhang (2017)
Assessment of genetic diversity and differentiation of Liposcelis bostrychophila (Psocoptera: Liposcelidae) in China using inter-simple sequence repeat (ISSR) fingerprinting
Applied Entomology and Zoology 52 (2), 241-246
Abstract: Liposcelis bostrychophila (Psocoptera: Liposcelidae) is a widely distributed pest that can cause considerable economic losses and pose human health risks. Rapid development of insecticide resistance has made L. bostrychophila increasingly difficult to control. To obtain information potentially useful for pest management, genetic diversity and differentiation of L. bostrychophila from five geographic locations in China was studied using inter-simple sequence repeat (ISSR). A total of 104 loci were found by ISSR markers and amplified using 9 selected primers. The percentage of polymorphic bands (PPB) was 91.4%. Shannon's information index (I) and Nei's gene diversity (He) indicated high genetic diversity at the species level. Population differentiation (Gst = 0.484)was average in these populations. Analysis of molecular variation (AMOVA) indicated that genetic variation was mainly distributed within populations. Gene flow (Nm = 0.534)was moderate. Cluster analysis showed that genotypes isolated from the same locations displayed higher genetic similarity and permitted the grouping of isolates of L. bostrychophila into three distinct clusters. The correlation between genetic distance and geographic distance was not significant.
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Research topic(s) for pests/diseases/weeds:
molecular biology - genes

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Liposcelis bostrychophila