Applied Entomology and Zoology (2000) 35, 245-249

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Kikuo Iwabuchi (2000)
A continuous cell line derived from larval fat bodies of Thysanoplusia intermixta (Lepidoptera: Noctuidae)
Applied Entomology and Zoology 35 (2), 245-249
Abstract: A continuous cell line has been established from larval fat bodies of a lepidopteran insect, Thysanoplusia intermixta. These cells were cultured in MGM-450 medium. The cell line, designated as TI-1, was a suspension of cells with a doubling time of 36 h and consisted mostly of spindle-shaped cells, although other shapes, notably spherical cells, also existed. The chromosome number of the cell line varied considerably with the mode of 150-179. This cell line was found to be susceptible to Autographa californica NPV (AcNPV). Isozyme analysis showed that the cells had distinctive patterns from other insect cell lines.
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Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Thysanoplusia intermixta Japan
Autographa californica nucleopolyhedrovirus (entomopathogen)