Applied Entomology and Zoology (2000) 35, 163-170

From Pestinfo-Wiki
Jump to: navigation, search

Tohru Hayakawa, Ei-ichi Shimojo, Masashi Mori, Masanori Kaido, Iwao Furusawa, Seiji Miyata, Yoshitaka Sano, Tsuguo Matsumoto, Yoshifumi Hashimoto and Robert R. Granados (2000)
Enhancement of baculovirus infection in Spodoptera exigua (Lepidoptera: Noctuidae) larvae with Autographa californica nucleopolyhedrovirus or Nicotiana tabacum engineered with a granulovirus enhancin gene
Applied Entomology and Zoology 35 (1), 163-170
Abstract: A baculovirus virulence-associated protein, enhancin, was engineered for production in a baculovirus-insect cell expression system and used for transformation of tobacco plants. A recombinant Autographa californica nucleopolyhedrovirus (AcNPV) with the enhancin gene from Trichoplusia ni granulovirus, AcEnh26, was propagated in Sf9 cells. The infected cultured cells combined with either AcNPV occlusion bodies (OBs) or Spodoptera exigua NPV (SeNpV) OBs were fed to 3rd stadium larvae of S. exigua. Feeding larvae with AcEnh26-infected cells resulted in a 21-fold and 10-fold enhancement of infection by AcNPV and SeNPV, respectively, compared to controls. When a similar feeding assay was performed with engineered tobacco plants, a 10-fold enhancement of AcNPV infection was observed, but a marked enhancement of SeNPV infection was not observed. Thus, the engineering and expression of the baculovirus enhancin gene in homologous and heterologous organisms allowed us to analyze its effects on NPV infection of larvae which demonstrated its viral enhancement function.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Full text of article

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
genetical engin./transgenic plants

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Spodoptera exigua
Autographa californica nucleopolyhedrovirus (entomopathogen) Spodoptera exigua
Spodoptera exigua multiple NPV (entomopathogen) Spodoptera exigua