Annals of the Entomological Society of America (1998) 91, 730-736

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A. Jeyaprakash, G. Lopez and M.A. Hoy (1998)
Extrachromosomal plasmid DNA transmission and amplification in Metaseiulus occidentalis (Acari : Phytoseiidae) transformants generated by maternal microinjection
Annals of the Entomological Society of America 91 (5), 730-736
Abstract: The plasmid pJKP2 (12.5 kb) was injected into Metaseiulus occidentalis females using a maternal microinjection procedure (Presnail and Hoy 1992). DNA transmission to their progenies was followed by the polymerase chain reaction (PCR) for several generations. After selecting and retaining individuals that tested positive by the PCR for 5 generations, individual females were used to initiate 12 separate transgenic lines (T1-T12). Southern blot analysis performed 10 generations after injection indicated that in at least 8 lines the plasmid DNA was transmitted extrachromosomally, and in 5 of these lines the extrachromosomal plasmid DNA was highly amplified with an estimated 10 million copies per female. The longevity, fecundity, immature mortality, and sex ratio of these transgenic lines were not different from the wild-type control under laboratory conditions. However, after 20 generations, few fransformants tested positive by the PCR and Southern blot analysis indicated no distinct banding pattern. Results from Southern blot analysis and the PCR indicated the extrachromosomal plasmid DNA was completely lost after 30 generations, suggesting a fitness cost did occur. Maternal microinjection delivers DNA efficiently to M. occidentalis and could be used for testing selectable markers or to deliver transposable-element vectors to obtain high rates of chromosomal integrations.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Ayyamperumal Jeyaprakash, Marjorie A. Hoy

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes
general biology - morphology - evolution

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Galendromus occidentalis (predator)