Annals of the Entomological Society of America (1996) 89, 576-580

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James K. Presnail and Marjorie A. Hoy (1996)
Maternal microinjection of the endoparasitoid Cardiochiles diaphaniae (Hymenoptera: Braconidae)
Annals of the Entomological Society of America 89 (4), 576-580
Abstract: Maternal microinjection is the injection of DNA into an adult female to deliver DNA to the eggs. Here we report the adaptation of this method to the parasitoid Cardiochiles diaphaniae (Hymenoptera: Braconidae). Results of preliminary dissections and injections with the plasmid pJKP2, followed by slot blot analysis, suggested that this method could be used to deliver injected DNA to the ovaries of this wasp. Subsequently, 16 adult females were injected, and allowed to produce offspring (the G0 generation). Polymerase chain reaction analysis of the next (G1) generation revealed that several individuals contained the plasmid sequences. In a 2nd experiment, the plasmid phsopd, containing the parathion hydrolase gene (opd ) of Pseudomonas diminuta, was injected. The plasmid probe hybridized to high molecular weight DNA from 3 of 38 G1 adults. A 4th adult produced a hybridization pattern consistent with integration of plasmid into the nuclear genome. The results indicate maternal microinjection can result in transformation of this parasitoid.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Marjorie A. Hoy

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution
genetical engin./transgenic plants

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Cardiochiles diaphaniae (parasitoid)