Annales de la Société Entomologique de France (2002) 38, 277-282

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Maha Mezghani Khemakhem, Hanem Makni and Mohamed Marrakchi (2002)
Identification par PCR-RFLP de marqueurs mitochondriaux chez deux espèces de Mayetiola nuisibles aux cultures de céréales (Diptera : Cecidomyiidae)
[PCR-RFLP identification of mitochondrial markers of Mayetiola (Diptera Cecidomyiidae) on cereals]
Annales de la Société Entomologique de France 38 (3), 277-282
Abstract: Mayetiola gall midges are major pests of cereals, causing every year important crop losses. In Tunisia and Morocco, two species, namely M. destructor (Say, 1817), and M. hordei Kieffer, 1909 were identified on the basis of electrophoretic studies. In order to develop a simple and reliable technique to distinguish the two species of gall midges, we have analysed the Restriction Fragment Length Polymorphism (RFLP) of the cytochrome b gene. There are three haplotypes called A, B and C among the samples of Mayetiola collected from barley and wheat in Tunisia. Using diagnostic alleles that are characteristic of the two species of Mayetiola, we have attributed the A haplotype to M. destructor and the B and C haplotypes to M. hordei.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: French)


Research topic(s) for pests/diseases/weeds:
identification/taxonomy
general biology - morphology - evolution


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Mayetiola destructor Wheat (Triticum) Tunisia
Mayetiola hordei Barley (Hordeum vulgare) Tunisia