Acta Entomologica Sinica (2006) 49, 137-141

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Zeng-Mei Li, Wen-Li Wang, Hui-Ming Wu, Shu-Sheng Liu, Yin-Quan Liu and Zhen-Hua Tang (2006)
Detection and analysis of spinosad residue in the body fluid of Cotesia plutellae larvae with HPLC
Acta Entomologica Sinica 49 (1), 137-141
Abstract: HPLC was used to investigate whether spinosad applied to parasitized host larvae could reach parasitoid larvae developing inside the hosts. Plutella xylostella larvae of a strain highly resistant to spinosad were used as hosts to rear the parasitoid Cotesia plutellae. When the parasitoids had developed to the stage of early instar larva, the host larvae were treated with 50 mg/L spinosad on the terga by topical application and then fed with cabbage leaves treated with 50 mg/L spinosad to allow the host larvae and the parasitoid larvae to develop. Three days after treatment, the parasitoid larvae inside the host were collected by dissecting the host larvae. The body fluid of P. xylostella and the bodies of parasitoid larvae were then analyzed with HPLC. Both of the active components, spinosyn A and spinosyn D, of spinosad were detected in the body fluid of host larvae and in the bodies of parasitoid larvae, and the residue concentrations were 2.79 mg/L and 0.94 mg/L in the host and the parasitoid respectively. The results demonstrate that spinosad applied to the host larvae can in turn reach inside the parasitoid larvae through penetration of cuticle and/or feeding of host hemolymph by the parasitoid larvae, and subsequently acts on the parasitoid larvae.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Shu-Sheng Liu

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
health/environmental effects of pesticides
Research topic(s) for beneficials or antagonists:
resistance to pesticides


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Plutella xylostella
Cotesia vestalis (parasitoid) Plutella xylostella