Acta Entomologica Sinica (2005) 48, 810-814

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Zheng-Xi Li and Ding-Xu Li (2005)
[Molecular identification of the primary and secondary symbionts of the green peach aphid, Myzus persicae (Sulzer)]
Acta Entomologica Sinica 48 (5), 810-814
Abstract: Aphids harbor not only primary endosymbionts, namely Buchnera aphidicola, but also different secondary symbionts. A single comigrating DNA band of about 1.5 kb was amplified from a population of the green peach aphid, Myzus persicae (Sulzer) collected from the experimental fields in China Agricultural University (Beijing) using a pair of 'universal' primers designed based on the full 16S rDNA of a wide range of eubacteria. This target DNA was then ligated to T-vector and cloned into E. coli DH5alpha. The clones were analyzed using restriction fragment length polymorphism (RFLP). The results showed that EcoRIcut the insert into two sub-fragments (~650 bp and ~850 bp) in all assayed samples, while double digestion with EcoRI and HindIII resulted in two different groups of RE (restriction enzyme) bands, with GroupIhaving only one EcoRI site but Group II having one EcoRI site and one HindIII site. Meanwhile, GroupIis dominant. Based on RE grouping, the full 16S rDNA representative sequences from different groups were sequenced, and the phylogenetic analysis demonstrated that GroupI belonged to Pantoea, closely related to P. agglomerans (homology: 99.70%), whereas GroupIIwas closely related to Buchnera aphidicola, the obligate symbiont of aphids (homology: 99.50%). This is the first report that Pantoea exists in aphids.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): Zheng-Xi Li

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Myzus persicae China (NE)