Acta Entomologica Sinica (2004) 47, 543-550

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Jiang Zhu, Song-Dong Shen, Wen-Bing Wang, Yu-Fang Zhu, Motoko Ikeda, Zhao-Li Hu and Ye Sheng (2004)
[Proliferation characteristics of three Japanese strains of Spodoptera litura multicapsid nucleopolyhedrovirus in cultural cells and sequence analysis of their polyhedrin genes]
Acta Entomologica Sinica 47 (5), 543-550
Abstract: Biological and molecular characteristics of three strains (K-3, G1-2 and G10-3) of the Spodoptera litura multicapsid nucleopolyhedrovirus (SpItMNPV), isolated from Honshu, Kyushu and Shikoku, Japan, which had strong pesticide activity to S. litura, were primarily studied using the cultured cell strain of S. litura. The polyhedrin genes of the three strains was cloned and sequenced. The results showed that: (1) the three Japanese strains K-3, G1-2 and G10-3 had different restriction fragmentation patterns and belonged to the nucleic acid type A, B and C of SpItMNPV respectively. (2) There are differences in the outputs of budded virus (BV) and polyhedron between the three strains. According to the analysis of immunological blotting, the molecular weights of polyhedrin in the three strains were different. (3) The polyhedrin gene of the Japanese SpItMNPV is composed of 747 nucleic acids, which codes a peptide of 249 amino acids. The nucleic acid sequences homology is as high as 98.9% compared with the Chinese strain, and 61.7% - 74.2% with other six NPVs compared. However, in the 5' upstream sequence (nt-1 - -100), SpItMNPV differs significantly from AcMNPV and BmNPV. There are 2 point mutations in the sequence with 8 highly conservative nucleic acids (nt-44 - -51), which is the domain of the promoter. Interestingly, in spite of only one mutation occurred in the polyhedron open reading frame of SpItMNPV C type, the western-blot result showed that the molecular weight of polyhedron of SpltMNPV C type is apparently different from that of A type and B type.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): Wenbing Wang

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes
general biology - morphology - evolution


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Spodoptera litura Japan
Spodoptera litura nucleopolyhedrovirus (entomopathogen) Spodoptera litura Japan