Acta Entomologica Sinica (2004) 47, 412-416

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Cai-Fu Jiang, Kai-Yu Liu, Rong Peng, Jian-Xin Peng and Hua-Zhu Hong (2004)
[Cloning and analysis of two Cry1Ac receptor-like aminopeptidase N cDNA fragments from Trichoplusia ni (Hübner) cultured cell]
Acta Entomologica Sinica 47 (3), 412-416
Abstract: Some aminopeptidase N (APNs) serve as Bacillus thuringiensis (Bt) Cry1Ac toxin receptor in the midguts of several lepidopteran insects. Two fragments of two different APN genes in BTI-TN-5B1-4 (a cloned cell line from Trichoplusia ni ovary) were amplified with two pairs of degenerate primers by RT-PCR, and the lengths of them were 188 by and 564 bp, and named AS188 (GenBank Accession: CD809324 ) and AS564 (GenBank Accession: CD809326 ) respectively. Deduced protein sequences were highly similar to that of several receptor APNs in insects, with sequence identities of 45% - 47% for the shorter one and 61% - 63% for the other.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): Hua-Zhu Hong

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Trichoplusia ni
Bacillus thuringiensis Cry1A-toxin (entomopathogen) Trichoplusia ni