Acta Entomologica Sinica (2002) 45, 743-747

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Dong-Fang Zhang (2002)
Purification and biological effects of a recombinant juvenile hormone esterase
Acta Entomologica Sinica 45 (6), 743-747
Abstract: The protein concentrations of cell supernatants infected with recombinant AcNPV (Autographa californica nulear polyhedrosis virus ) containing wild type or mutant juvenile hormone esterase were between 153.2 - 188.0 µg/mL. The enzyme activity recovery rate was about 33%. The stepwise purification of the enzyme had similar results as the gradient method, but was more convenient for handling large volumes of cell supernatants. The cuticle blackening test showed that the recombinant juvenile hormone esterase from Heliothis virescens had biological effects on tobacco horn moth larvae (Manduca sexta).
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
genetical engin./transgenic plants

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Autographa californica nucleopolyhedrovirus (entomopathogen)