Acta Entomologica Sinica (2002) 45, 693-695

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Ying-Chun Zhang, Bo Liu, Zhe-Min Zheng and Li Li (2002)
DNA extraction and RAPD-PCR of insect specimens preserved with different methods
Acta Entomologica Sinica 45 (5), 693-695
Abstract: Genomic DNA were extracted and amplified using the polymerase chain reaction and arbitrary primers from specimens of seven insect species: Chrysomela vigintipunctata (Scopoli) (Coleptera : Chrycomelidae), Harmonia axyridis Pallas and Coccinella septempunctata Linnaeus (Coleoptera: Coccinellidae), Agrotis ypsilon (Rottemberg) (Lepidoptera: Noctuidae), Crocothemis servilia Drury (Odonata: Libellulidae), and Oxya abentata Willemse and O. chinensis (Thunberg) (Orthoptera: Catantopidae). Of the specimens assayed, some had been naturally dried, some oven-dried and the remainder had been preserved in alcohol. The results suggest that, with respect to DNA extraction, oven-dried specimens and those preserved in alchohol are better than naturally dried specimens. Extraction of DNA extraction using the CTAB method was easier, and more economical than other methods.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)


Research topic(s) for pests/diseases/weeds:
molecular biology - genes
Research topic(s) for beneficials or antagonists:
molecular biology - genes


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Agrotis ipsilon
Oxya chinensis
Chrysomela vigintipunctata
Coccinella septempunctata (predator)
Harmonia axyridis (predator)