Acta Entomologica Sinica (2001) 44, 276-280

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Hua-Zhu Hong and Jian-Xin Peng (2001)
Establishment of an insect cell line for high level expression of recombinant protein
Acta Entomologica Sinica 44 (3), 276-280
Abstract: A new cell line was established from Trichoplusia ni fatbody in Excell 400 medium supplemented with 5% FBS. The maximum cell density reached 2.2 x 106 cells/mL and the population doubling time was 22.9 h for this cell line. The cell line was highly sensitive to AcMNPV, and produced 86 occlusion bodies per cell. The expression levels for two recombinant proteins, ß-galactosidase and alkaline phosphatase, in this cell line were compared to that in IPLB-Sf-21 and BTI-5B1 . The activities of ß-galactosidase and alkaline phosphatase from the novel cell line were (225.5 ± 13.4) IU/mL and (4.7 ± 0.61) IU/mL respectively. The expression levels from the fat body cells and from BTI-TN-5B1, which is known to express recombinant proteins at the highest levels, were almost the same and the former was a little bit better. The new cell line can efficiently support the expression of foreign protein and is designated HNU-Tn-FB1.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)


Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Trichoplusia ni
Autographa californica nucleopolyhedrovirus (entomopathogen)