Oryctes rhinoceros nudivirus (entomopathogen)

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Rods (R) of the Oryctes rhinoceros nudivirus in the nucleus of a fatbody cell of a rhinoceros beetle larva (NM = nuclear membrane).
Author: Alois Huger

Oryctes rhinoceros nudivirus (OrNV)

This virus is the most important biological control agent of the Indian rhinoceros beetle (Oryctes rhinoceros), a serious coconut pest in tropical parts of Asia and the Pacific. It is either naturally present in the beetle populations or has been deliberately released in various countries. The virus infects adults and larval stages and the adults spread the disease readily within the pest populations. The virus particles are rod-shaped and enveloped, about 220 x 120 nm large. The genome consists of a large dsDNA molecule with about 128 kb and 139 open reading frames (Wang et al., 2011). The virus is the type species of the genus Alphanudivirus. The genus is related to the baculoviruses but is included in a separate family. A review on the biology of this virus has been provided by Huger, 2005.

Discovery and biology of the virus

Oryctes rhinoceros is a large beetle (up to 5 cm in length) which is found in southern Asia and some Pacific islands. The adult beetle bores into the growing fronds of coconut and oil palms, causing serious damage. The beetles breed in dead palm trunks, where the larvae feed on decaying wood.

Top: Adult male rhinoceros beetle (Oryctes rhinoceros). Bottom: Two third instar larvae, left with symptoms of virus infection (note prolapsed rectum and transparent coloration), right healthy larva.
Author: Alois Huger

The Oryctes rhinoceros nudivirus was discovered by A.M. Huger in the early 1960s in Malaysia while searching for O. rhinoceros diseases which could be used in biological control programs against this pest (Huger, 1966a, Huger, 1966b and Huger, 1973). He found that larvae and adult beetles become infected through the gut. Infected larvae become transparent, often with a prolapsed rectum, and 3rd instar larvae die after about 3-4 weeks. Adults survive for a similar length of time and can still fly and visit palms and breeding places for 2-3 weeks.

Both adults and larvae infect each other during feeding or by small quantities of the virus getting accidentally into their mouth. They both excrete virus particles after they have become infected. This is particularly clear in adult beetles where the infected midgut becomes completely filled with virus particles, swells and turns white. The gut symptoms of adults provide a simple way of diagnosing infected adults in the field, even without a dissecting microscope. By continuously excreting virus particles, the infected beetles spread the disease by contaminating breeding places and coconut palms with the virus (see below for more details on the infection process).

Synonyms:
Baculovirus oryctes
Rhabdionvirus oryctes

Introductions and natural distribution of the virus

The virus disease was initially transferred from Malaysia to Samoa and released there by placing infected larvae in breeding places. It quickly spread around the islands of Samoa, causing a significant decline in the beetle populations and the damage on coconut palms. It was subsequently also released in other Pacific countries where O. rhinoceros occurs, that is Fiji, Tonga, Wallis Island, Tokelau Islands and Papua and New Guinea. In addition, it was later released on the atolls of the Maldives in the Indian Ocean and was even introduced into the Oryctes monoceros populations of Tanzania and the Seychelles. Releasing a few virus infected adult beetles is the simplest method of introducing the disease into a population. Apart from Malaysia the virus was found to be already naturally present in the Philippines, Indonesia and India.

Sampling the percentage of virus-infected adult beetles after its introduction showed clearly the importance of the virus as a natural control agent. In Samoa, around 35% of the adult beetles collected in the field were infected a few years after the introduction (Zelazny, 1973). In the Maldives, the average infection rate among field collected beetles was 29%, 46% and 41% at different times after the releases, that is after 1-6 months, 6-12 months and > 12 months (Zelazny et al., 1990). The epidemiology of the virus depends on a number of environmental factors like the density of the beetle population, the amount of breeding places and the density of coconut palms. In addition, 4 different virus strains/isolates were released in the Maldives on a total of 17 islands.

Surveys in the Philipinnes and Indonesia have given a mixed picture on the natural prevalence of the virus disease in the native range of O. rhinoceros. Adult beetles collected in the Philippines, Borneo (Kalimantan) and parts of Sumatra and Sulawesi had an infection rate (20-30%) comparable to that observed after the virus introductions in the Pacific and the Maldives. However, a very low infection rates were found in an oil palm plantation in Sumatra, indicating that under special environmental conditions the virus transmission does not work well. In Java and South Sulawesi the virus was intially thought to be absent but was later found to occur at extremely low levels, suggesting that in some populations resistance to the virus had developed (see Zelazny, 1977 and Zelazny et al., 1989).

Still, releases of virus-infected beetles in Central Java reduced the O. rhinoceros populations. Eleven years after these releases, the beneficial effects were still visible in the palm damage of the treated plots. However, strangely, the damage reduction had not spread to the untreated control plots, 10-20 km away (Munaan et al., 1989). At the same time there was evidence, that different strains of the virus had different impacts on the beetle population (Zelazny, 1979 and Zelazny et al., 1990). Different methods of infecting adult beetles and preparing a virus inoculum have been developed (Zelazny et al., 1985 and 1987). However, a limiting factor for releasing additional virus-infected beetles into a beetle population is usually the availability of adults which are difficult to collect from coconut palms.

Diagram of the ecology of Oryctes rhinoceros and its virus disease during a replanting situation. Six adult beetles searching for breeding places (dotted lines = flight paths) are shown and 2 of them are infected with the virus disease. Above the beetles are 8 coconut trunks lying on the ground, one of them with larvae. Because of the abundance of possible breeding places there is no contact between the infected and healthy beetles or the larvae and no virus transmission. Below the beetles are a pile of coconut trunks hidden from the beetles by cover crop and 3 dead-standing palms (one with larvae). The dead-standing palms are the preferred breeding sites and there is frequent contact between the beetles and therefore virus transmission. - (Click on image to enlarge it)
.

Ecology of the virus and management options

Form surveys in the Philippines and Indonesia it became clear that the transmission of the virus disease and its impact on the O. rhinoceros population depends very much on the environmental conditions. Coconut palms which had died without being felled (dead-standing palms) were most often used by the beetles for breeding and also had the highest disease incidence. Stumps and logs lying on the ground were less often used and had a much lower disease incidence (see Zelazny & Alfiler, 1989 as well as Zelazny et al., 1992). The dead-standing palms are the original type of breeding site used by O. rhinoceros before plantations existed and coconut trees were felled. They are obviously recognized most readily by the beetles as a suitable breeding place. Logs on the ground are less often found by the beetles, partly because they are often hidden by vegetation.

Transmission of the virus occurs through contact between adult beetles as well as between adults and larvae, in breeding sites as well as in coconut palms. The more often a specific breeding place is visited by adult beetles the greater the chance of virus transmission. Thus, some dead-standing palms per hectare with few other available breeding places in the vicinity are the ideal conditions for virus transmission, resulting in little damage to coconut palms. On the other hand, when coconut or oil palm plantations are replanted, an environment is created which blocks transmission of the virus. There are simply too many possible breeding places and little contact among adults and between adults and larvae. This is illustrated in the top part of the diagram on the right. The bottom part illustrates how the transmission of the virus can be improved under replanting conditions by hiding the breeding places. Cover crop is grown over the felled trunks and around 5 palms per hectare are left dead standing.

In conclusion, management of Oryctes involves:

  1. Promoting the spread of the virus during replanting situations by hiding the felled trunks under cover crops and leaving a few palms dead standing.
  2. If there are indications that the O. rhinoceros population has developed resistance to the virus (like in Java), additional releases of a virulent virus strain might be beneficial. However, judging from the results of such releases in Java, the damage reduction might be localized.
Epithelium of healthy adult midgut showing regenerative crypts (R). - (Click on image to enlarge it)
Author: Alois Huger

Microscopic details of virus infections in O. rhinoceros

In adult beetles, the virus infects mainly the midgut and only multiplies in the cell nuclei. In contrast to the baculoviruses which are embedded in a crystalline protein matrix, the Oryctes rhinoceros virus does not produce such protein crystals, therefore the name "nudivirus". In rhinoceros beetle larvae various other tissues can become infected by the virus apart from the gut, but again the virus only multiplies in the cell nuclei.

The midgut wall of the beetle is composed of a single layer of cells. If gut cells are damaged or die, they are replaced by new cells which are generated in the "regenerative crypts" (see illustration at left). When beetles become infected by the virus this replacement mechanism is accelerated and the dying cells are pushed into the inside of the gut. The gut becomes swollen and completely filled with dead cells, mainly composed of nuclei filled with virus particles. Eventually the virus particles are excreted by the infected beetle. For more details see the light - and electron microscopic images below and Huger, 1973.