Mycologia (2004) 96, 781-798

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Jan M. van Niekerk, Pedro W. Crous, J.Z. Ewald Groenewald, Paul H. Fourie and Francois Halleen (2004)
DNA phylogeny, morphology and pathogenicity of Botryosphaeria species on grapevines
Mycologia 96 (4), 781-798
Abstract: Several species of Botryosphaeria are known to occur on grapevines, causing a wide range of disorders including bud mortality, dieback, brown wood streaking and bunch rot. In this study the 11 Botryosphaeria spp. associated with grapevines growing in various parts of the world, but primarily in South Africa, are distinguished based on morphology, DNA sequences (ITS-1, 5.8S, ITS-2 and EF1-alpha) and pathological data. Botryosphaeria australis, B. lutea, B. obtusa, B. parva, B. rhodina and a Diplodia sp. are confirmed from grapevines in South Africa, while Diplodia porosum, Fusicoccum viticlavatum and F. vitifusiforme are described as new. Although isolates of B. dothidea and B. stevensii are confirmed from grapevines in Portugal, neither of these species occurred in South Africa, nor were any isolates of B. ribis confirmed from grapevines. All grapevine isolates from Portugal, formerly presumed to be B. ribis, are identified as B. parva based on their EF1-alpha sequence data. From artificial inoculations on grapevine shoots, we conclude that B. australis, B. parva, B. ribis and B. stevensii are more virulent than the other species studied. The Diplodia sp. collected from grapevine canes is morphologically similar but phylogenetically distinct from D. sarmentorum. Diplodiasarmentorum is confirmed as anamorph of Otthia spiraeae, the type species of the genus Otthia (Botryosphaeriaceae). A culture identified as O. spiraeae clustered within Botryosphaeria and thus is regarded as probable synonym. These findings confirm earlier suggestions that the generic concept of Botryosphaeria should be expanded to include genera with septate ascospores and Diplodia anamorphs.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Pedro W. Crous, Johannes Z. Groenewald

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Lasiodiplodia theobromae Grapevine (Vitis) South Africa
Diplodia mutila Grapevine (Vitis) Portugal (continental)
Botryosphaeria (genus)
Botryosphaeria dothidea Grapevine (Vitis) Portugal (continental)
Diplodia seriata Grapevine (Vitis) South Africa
Neofusicoccum ribis
Neofusicoccum parvum Grapevine (Vitis) Portugal (continental)
Neofusicoccum parvum Grapevine (Vitis) South Africa
Neofusicoccum luteum Grapevine (Vitis)
Neofusicoccum australe Grapevine (Vitis) South Africa