Japanese Journal of Applied Entomology and Zoology (2011) 55, 254-257

Ryouichi Takeuchi and Satoshi Toda (2011)
[Discrimination of two reproductive forms of Thrips tabaci by PCRRFLP, and distribution of arrhenotokous T. tabaci in Tottori Prefecture]
Japanese Journal of Applied Entomology and Zoology 55 (4), 254-257
Abstract: To discriminate between arrhenotokous and thelytokous forms of the onion thrips (Thrips tabaci), we developed a simple molecular diagnostic method that uses PCR-RFLP of the mitochondrial cytochrome c oxidase subunit I (COI) gene. Digestion of 490-bp COI amplicons with EcoO109I provided reproductive form-specific RFLP patterns. Using this method, we surveyed the frequencies of the two reproductive forms in 9 Welsh onion fields in Tottori Prefecture. Arrhenotoky dominated in the western area, although 100% of individuals were thelytokous in the eastern area. The COI haplotype of the arrhenotokous individuals was haplotype 1 or 2, which has not previously been found in Japan.
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(original language: Japanese)
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Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Thrips tabaci		Japan