European Journal of Plant Pathology (2012) 132, 9-16

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Zhixiang Zhang, Shan Peng, Dongmei Jiang, Song Pan, Hongqing Wang and Shifang Li (2012)
Development of a polyprobe for the simultaneous detection of four grapevine viroids in grapevine plants
European Journal of Plant Pathology 132 (1), 9-16
Abstract: This study aimed to develop a polyprobe for the simultaneous detection of four viroids that infect grapevine: Hop stunt viroid (HSVd), Australian grapevine viroid (AGVd), Grapevine yellow speckle viroid-1 and 2 (GYSVd-1, 2), using a non-isotopic dot blot hybridization technique. A polyprobe was constructed by cloning tandem full-length sequences of HSVd, AGVd and GYSVd-1 into a single vector. The cRNA polyprobe detected all four viroids with similar sensitivity to that obtained using individual probes. In addition, samples of 78 varieties from Beijing and Xinjiang were analyzed using the polyprobe to survey the incidence of grapevine viroids in China. The result demonstrated that grapevine viroids were detected in 56 (71.8%) varieties. In this study, a rapid, reliable and cost-effective approach to the simultaneous detection of four grapevine viroids has been developed which has the potential for routine use in quarantine and certification programs.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Shi-Fang Li

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution
identification/taxonomy


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Hop stunt viroid Grapevine (Vitis)
Grapevine yellow speckle viroid 1 Grapevine (Vitis)
Australian grapevine viroid Grapevine (Vitis)
Grapevine yellow speckle viroid 2 Grapevine (Vitis)