Acta Phytopathologica et Entomologica Hungarica (2010) 45, 1-11

A. Almási, I. Tóbiás, I.N. Manoussopoulos, Zs. Basky and L. Palkovics (2010)
Sequence comparison of the HC-Pro and CP proteins of two Potato virus Y strains differing in aphid transmission and systemic movement
Acta Phytopathologica et Entomologica Hungarica 45 (1), 1-11
Abstract: Nucleotide and amino acid sequences of the helper component protease (HC-Pro) and the coat protein (CP) of two Hungarian Potato virus Y (PVY) isolates, differing in aphid transmissibility were determined. Isolate PVY-5 belongs to the common "O" strain (PVY ^O ), whereas isolates PVY-98 and PVY-111 belong to the "N" (PVY ^N ) and the PVY-NTN and PVY-H to the "NTN" (PVY ^NTN ) strains, respectively. The PVY-5 isolate varied significantly from the others in aphid transmission and in the ability to systemically infect potato plants. To elucidate whether these differences were due to mutations affecting known functional motifs, the corresponding cistrons of the two proteins were sequenced and aligned. Our analysis showed that none of the well-known motifs, responsible for aphid transmission in the two proteins had been affected. However, the defective isolate had two natural mutations in the HC-Pro in the vicinity of the PTK motif, and a number of mutations in the CP, distributed both in the N-terminus and the central region. As these two proteins are the only known viral participants in the aphid transmission mechanism, it is likely that some of the observed mutations might be involved in this process. Thus, our results indicate that other, previously unidentified sequences or factors may influence virus-vector interactions and transmission of PVY.
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Research topic(s) for pests/diseases/weeds:
molecular biology - genes
transmission/dispersal of plant diseases

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Potato virus Y		Hungary